WebMay 1, 1995 · This system allowed for the recovery of putative transformants within four weeks after co-cultivation of tissue samples with Agrobacterium. WebSep 3, 2001 · mrpJ, the last of nine genes of the mrp operon, encodes a 107 amino acid protein that contains a putative helix–turn–helix domain. ... One of many ampicillin-resistant and kanamycin-resistant transformants (as a result of a single crossover) was selected and then grown in nonselective medium to allow for a second crossover.
Martha Malapi, Ph.D. - Branch Chief - LinkedIn
WebTransformation efficiency is 103 to 104 transformants per μg of linearized DNA. Note: Spheroplasting is not recommended for Pichia vectors with an antibiotic resistance marker. Damage to the cell wall leads to increased sensitivity to the antibiotic, causing putative transformants to die before they express the antibiotic resistance gene. WebIt is found that mRNA sequences of the endogenous glucanase glb gene with varying degrees of homology to the Nicotiana plumbaginifolia gn1 transgene are targeted by the silencing machinery, although less efficiently than correspondingTransgene regions. Post‐transcriptional gene silencing (PTGS) is characterized by the accumulation of short … lindsay lohan among the shadows poster
The Selection and Analysis of Transformants - Plant Transgenics
WebMar 9, 2016 · Putative transformants were regenerated on selection-free medium using our previously established in vitro regeneration method. Explant type, genotype, effect of pre … WebTwo genes, KlARO8.1 and KlARO8.2, were found to be responsible for L-methionine aminotransferase activity. Transformants carrying these genes cloned in the pCXJ10 vector produced threefold-larger amounts of VSCs than the transformant containing the plasmid without any insert or other related putative aminotransferases produced. WebThe PCR results revealed the presence of glyoxalase I gene in the transformants. Original language: English: Pages (from-to) 203-210: Number of pages: 8: Journal: ... (0.3 mgl-1) for root formation and the plantlets were hardened in plastic cups with sterile soil. The putative transgenic plantlets were analyzed histochemically for gus gene and ... lindsay lohan and chad michael murray