Flashplate assay
WebFlashPlate® methylation assays, long term cell proliferation assays and symmetric dimethylation of known cellular protein SmD3 were used to assess in vitro potency and functional effect of PRMT5 inhibition. A number of compounds from two different series showed strong in vitro WebSubstrates FlashPlate assay: H4 (1-15)-Biotin, 40 nM SAM, 1 µM Assay Properties Reaction S-adenosyl-L- [methyl-³H]methionine + histone L-arginine = S-adenosyl-L-homocysteine + histone [methyl-³H]-L- arginine …
Flashplate assay
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WebJan 3, 2024 · In this four-day polarization assay, ... 180 μL of 20 mM Tris buffer (pH 8.0) were added, and the mixture was transferred to a 96-well FlashPlate and incubated for 1 h. The counts per minute (CPM ... WebA FlashPlate assay for the identification of PARP-1 inhibitors A novel FlashPlate scintillation proximity assay has been developed for the high-throughput screening …
WebProtein-Ligand Binding and Molecular Recognition Database. Affinity Data IC50: 5.50E+3nM Assay Description: The kinase assay is performed as 384-well flashplate assay.1 nM IKKε, 800 nM biotinylated IκBα(19-42) peptide (biotin-C6-C6-GLKKERLLDD... More data for this Ligand-Target Pair WebThe NS1 protein is a nonstructural protein encoded by the influenza A virus. It is responsible for many alterations produced in the cellular metabolism upon infection by the virus and for modulation of virus virulence. The NS1 protein is able to
WebAffinity Data IC50: 5.50E+3nM Assay Description: The experimental batches are carried out in a flashplate system with 384 wells/microtitration plate.In each case, the PDK1 ... 5.50E+3nM Assay Description: The kinase assay is performed as 384-well flashplate assay.0.6 nM TANK binding kinase (TBK1), 800 nM biotinylated MELK-derived peptide ... WebMar 15, 2024 · This assay consists of three steps: (1) PARP-1-initiated synthesis of the hyperbranched PAR polymer, (2) formation of the AuNPs-dsDNA-ALP nanostructures, and (3) ALP-catalyzed dephosphorylation of AMPPD for generating chemiluminescence signal. ... A FlashPlate assay for the identification of PARP-1 inhibitors. J. Biomol. Screen. …
WebMar 1, 2001 · The resultant 96-/384-well microplate assays are suitable for primary HTS, hit selectivity determination, and/or elucidating the mechanism of action of inhibitors. In all …
WebAdenylyl Cyclase Activation Flashplate Assay, supplied by PerkinElmer, used in various techniques. Bioz Stars score: 86/100, based on 1 PubMed citations. ZERO BIAS - … djed columnWebJun 1, 2003 · The assay was originally developed for the 96-well FlashPlate but is easily transferred to a 384-well format. Moreover, the authors demonstrate that the assay is … djed coinWebFlashPlate-based Protein Kinase Assay Protocol (33PanQinase® Assay) Standard Procedure for all kinases (Manual Assay) Materials: ATP (e.g. Sigma, # A-7699) CaCl 2 … djed crypto priceWebThe assay can also be performed in FlashPlates or as a filtration assay using a filter plate. The SPA and FlashPlate formats are homogeneous formats, which means no wash steps are required. In the filtration assay, you will wash away unbound GTP using a vacuum manifold or cell harvester. djed cotiWebFlashPlate scintillation proximity assays for characterization and screening of DNA polymerase, primase, and helicase activities. DNA replication proteins represent a class … djed božićnjak poziv konzumWebTo optimize the DNMT1 Flashplate™ assay, we performed an enzymatic titration and studied the kinetics of the reaction at 37°C and at room temperature to limit evaporation. As 90 nM of enzyme gave the highest signal in the linear response range ( Supplementary Figure S2A ), it was chosen as concentration for the assay. djed cryptoWeb2 FlashPlate Assay, 2 SMP003 125I, 96-well with red-emitting scintillant, for solid-phase radiobinding assays in ViewLuxTM Imager Basic Image FlashPlate-384, white, 5 RMP100E shallow 384-well 20 RMP100 Streptavidin Image FlashPlate-384 5 RMP110E 40 RMP110 filter plates for receptor binding assays and cell harvesting djed egipcio